Somatostatin regulates intracellular signaling in human carotid endothelial cells.
Publication/Presentation Date
7-9-2004
Abstract
Somatostatin (somatotropin release inhibitory factor; SRIF) is an endogenous peptide produced at sites of inflammation, making the SRIF a candidate in regulating vascular inflammation. We have used primary human coronary artery endothelial cells (hCAEC) as a model to study SRIF's vascular actions. RT-PCR analysis of hCAEC total mRNA demonstrated the presence of the sst(4) receptor subtype, providing a target for SRIF intracellular signaling. Western blotting with phospho-specific ERK1/2 antibodies showed that SRIF-14 acutely inhibited basal phosphorylation of the extracellular regulated kinases (ERK1/2) by 80%. In addition, SRIF-14 treated hCAEC cell lysates showed a 2.6-fold increase in phosphatase activity, which was inhibited by sodium vanadate. Furthermore, SRIF-14 appeared to be anti-inflammatory in hCAEC as IL-1beta-induced adhesion molecule expression was reduced by 50%. Together, these results show that the coronary artery endothelium is a direct target of SRIF action.
Volume
319
Issue
4
First Page
1222
Last Page
1227
ISSN
0006-291X
Published In/Presented At
Badway, A. C., West, F. M., Tente, S. M., & Blake, A. D. (2004). Somatostatin regulates intracellular signaling in human carotid endothelial cells. Biochemical and biophysical research communications, 319(4), 1222–1227. https://doi.org/10.1016/j.bbrc.2004.05.110
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
15194497
Department(s)
Administration and Leadership
Document Type
Article