Aneurysmal lesions of patients with abdominal aortic aneurysm contain clonally expanded T cells.
Publication/Presentation Date
5-15-2014
Abstract
Abdominal aortic aneurysm (AAA) is a common disease with often life-threatening consequences. This vascular disorder is responsible for 1-2% of all deaths in men aged 65 years or older. Autoimmunity may be responsible for the pathogenesis of AAA. Although it is well documented that infiltrating T cells are essentially always present in AAA lesions, little is known about their role in the initiation and/or progression of the disease. To determine whether T cells infiltrating AAA lesions contain clonally expanded populations of T cells, we amplified β-chain TCR transcripts by the nonpalindromic adaptor-PCR/Vβ-specific PCR and/or Vβ-specific PCR, followed by cloning and sequencing. We report in this article that aortic abdominal aneurysmal lesions from 8 of 10 patients with AAA contained oligoclonal populations of T cells. Multiple identical copies of β-chain TCR transcripts were identified in these patients. These clonal expansions are statistically significant. These results demonstrate that αβ TCR(+) T lymphocytes infiltrating aneurysmal lesions of patients with AAA have undergone proliferation and clonal expansion in vivo at the site of the aneurysmal lesion, in response to unidentified self- or nonself Ags. This evidence supports the hypothesis that AAA is a specific Ag-driven T cell disease.
Volume
192
Issue
10
First Page
4897
Last Page
4912
ISSN
1550-6606
Published In/Presented At
Lu, S., White, J. V., Lin, W. L., Zhang, X., Solomides, C., Evans, K., Ntaoula, N., Nwaneshiudu, I., Gaughan, J., Monos, D. S., Oleszak, E. L., & Platsoucas, C. D. (2014). Aneurysmal lesions of patients with abdominal aortic aneurysm contain clonally expanded T cells. Journal of immunology (Baltimore, Md. : 1950), 192(10), 4897–4912. https://doi.org/10.4049/jimmunol.1301009
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
24752442
Department(s)
Administration and Leadership
Document Type
Article