Urine Extracellular Vesicle GATA2 mRNA Discriminates Biopsy Result in Men with Suspicion of Prostate Cancer.

Authors

• J Woo
• S Santasusagna
• J Banks
• S Pastor-Lopez
• K Yadav
• M Carceles-Cordon
• A Dominguez-Andres
• R B Den
• L R Languino
• R Pippa
• C D Lallas
• G Lu-Yao
• W K Kelly
• K E Knudsen
• V Rodriguez-Bravo
• A K Tewari
• J M Prats
• B E Leiby
• L G Gomella
• Josep Domingo-Domenech

Publication/Presentation Date

10-1-2020

Abstract

PURPOSE: Prostate specific antigen has limited performance in detecting prostate cancer. The transcription factor GATA2 is expressed in aggressive prostate cancer. We analyzed the predictive value of urine extracellular vesicle GATA2 mRNA alone and in combination with a multigene panel to improve detection of prostate cancer and high risk disease.

MATERIALS AND METHODS: GATA2 mRNA was analyzed in matched extracellular vesicles isolated from urines before and after prostatectomy (16) and paired urine and tissue prostatectomy samples (19). Extracellular vesicle GATA2 mRNA performance to distinguish prostate cancer and high grade disease was tested in training (52) and validation (165) cohorts. The predictive value of a multigene score including

RESULTS: Confirming its prostate origin, urine extracellular vesicle GATA2 mRNA levels decreased significantly after prostatectomy and correlated with prostate cancer tissue GATA2 mRNA levels. In the training and validation cohort GATA2 discriminated prostate cancer (AUC 0.74 and 0.66) and high grade disease (AUC 0.78 and 0.65), respectively. Notably, the GAPT-E score improved discrimination of prostate cancer (AUC 0.84 and 0.72) and high grade cancer (AUC 0.85 and 0.71) in both cohorts when compared with each biomarker alone and PT-E (

CONCLUSIONS: Urine extracellular vesicle GATA2 mRNA analysis improves the detection of high risk prostate cancer and may reduce the number of unnecessary biopsies.

Volume

204

Issue

4

First Page

691

Last Page

700

ISSN

1527-3792

Published In/Presented At

Woo, J., Santasusagna, S., Banks, J., Pastor-Lopez, S., Yadav, K., Carceles-Cordon, M., Dominguez-Andres, A., Den, R. B., Languino, L. R., Pippa, R., Lallas, C. D., Lu-Yao, G., Kelly, W. K., Knudsen, K. E., Rodriguez-Bravo, V., Tewari, A. K., Prats, J. M., Leiby, B. E., Gomella, L. G., & Domingo-Domenech, J. (2020). Urine Extracellular Vesicle GATA2 mRNA Discriminates Biopsy Result in Men with Suspicion of Prostate Cancer. The Journal of urology, 204(4), 691–700. https://doi.org/10.1097/JU.0000000000001066

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

32250729

Department(s)

Administration and Leadership

Document Type

Article