Lymphotoxin-α is a novel adiponectin expression suppressor following myocardial ischemia/reperfusion.
Publication/Presentation Date
3-15-2013
Abstract
Recent clinical observations demonstrate adiponectin (APN), an adipocytokine with potent cardioprotective actions, is significantly reduced following myocardial ischemia/reperfusion (MI/R). However, mechanisms responsible for MI/R-induced hypoadiponectinemia remain incompletely understood. Adult male mice were subjected to 30-min MI followed by varying reperfusion periods. Adipocyte APN mRNA and protein expression and plasma APN and TNFα concentrations were determined. APN expression/production began to decline 3 h after reperfusion (reaching nadir 12 h after reperfusion), returning to control levels 7 days after reperfusion. Plasma TNFα levels began to increase 1 h after reperfusion, peaking at 3 h and returning to control levels 24 h after reperfusion. TNFα knockout significantly increased plasma APN levels 12 h after reperfusion but failed to improve APN expression/production 72 h after reperfusion. In contrast, TNF receptor-1 (TNFR1) knockout significantly restored APN expression 12 and 72 h after reperfusion, suggesting that other TNFR1 binding cytokines contribute to MI/R-induced APN suppression. Among many cytokines increased after MI/R, lymphotoxin-α (LTα) was the only cytokine remaining elevated 24-72 h after reperfusion. LTα knockout did not augment APN levels 12 h post-reperfusion, but did so by 72 h. Finally, in vitro treatment of adipocytes with TNFα and LTα at concentrations seen in MI/R plasma additively inhibited APN expression/production in TNFR1-dependent fashion. Our study demonstrates for the first time that LTα is a novel suppressor of APN expression and contributes to the sustained hypoadiponectinemia following MI/R. Combining anti-TNFα with anti-LTα strategies may achieve the best effects restoring APN in MI/R patients.
Volume
304
Issue
6
First Page
661
Last Page
667
ISSN
1522-1555
Published In/Presented At
Lau, W. B., Zhang, Y., Zhao, J., Liu, B., Wang, X., Yuan, Y., Christopher, T. A., Lopez, B., Gao, E., Koch, W. J., Ma, X. L., & Wang, Y. (2013). Lymphotoxin-α is a novel adiponectin expression suppressor following myocardial ischemia/reperfusion. American journal of physiology. Endocrinology and metabolism, 304(6), E661–E667. https://doi.org/10.1152/ajpendo.00012.2013
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
23360826
Department(s)
Administration and Leadership
Document Type
Article