Vaccination with a genetically modified Brugia malayi cysteine protease inhibitor-2 reduces adult parasite numbers and affects the fertility of female worms following a subcutaneous challenge of Mongolian gerbils (Meriones unguiculatus) with B. malayi infective larvae.

Authors

• Sridhar Arumugam
• Junfei Wei
• Danielle Ward
• David Abraham
• Sara Lustigman
• Bin Zhan
• Thomas R Klei

Publication/Presentation Date

9-1-2014

Abstract

Vaccination of Mongolian gerbils with Brugia malayi cysteine protease inhibitor-2 in which the amino acid Asn66 was mutated to Lys66 (Bm-CPI-2M) resulted in reduced parasite numbers of 48.6% and 48.0% at 42 and 90 days p.i. with B. malayi L3s. Fertility of female worms was also affected at 90 days p.i. In vitro killing of L3s observed in the presence of gerbil peritoneal exudate cells and anti-Bm-CPI-2M sera suggests antibody-dependent cell-mediated cytotoxicity as a putative protective mechanism. These observations suggest that Bm-CPI-2M is a promising prophylactic and anti-fecundity vaccine candidate.

Volume

44

Issue

10

First Page

675

Last Page

679

ISSN

1879-0135

Published In/Presented At

Arumugam, S., Wei, J., Ward, D., Abraham, D., Lustigman, S., Zhan, B., & Klei, T. R. (2014). Vaccination with a genetically modified Brugia malayi cysteine protease inhibitor-2 reduces adult parasite numbers and affects the fertility of female worms following a subcutaneous challenge of Mongolian gerbils (Meriones unguiculatus) with B. malayi infective larvae. International journal for parasitology, 44(10), 675–679. https://doi.org/10.1016/j.ijpara.2014.05.003

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

24929131

Department(s)

Administration and Leadership

Document Type

Article