IL-12 eliminates the Th-2 dependent protective immune response of mice to larval Strongyloides stercoralis.
Publication/Presentation Date
1-1-1997
Abstract
The goal of the present study was to determine if immune-mediated killing of S. stercoralis L3 in mice could be modulated by shifting from a Th-2 to a Th-1 type immune response. L3 killing in immunized mice was ablated in CD4+ T cell-depleted animals, but not in CD8+ T cell-depleted or beta 2-microglobulin-deficient mice. Treatment of immunized mice with IL-4 or IL-5 neutralizing MoAb significantly reduced the protective effects of vaccination against S. stercoralis, while protective immunity was unimpaired in IFN-gamma knockout mice. Recombinant IL-12 was administered to infected mice to switch the immune response from a Th-2 to a Th-1 type response. Protective immunity was ablated in immunized mice that received IL-12 therapy. Eosinophil numbers, eosinophil peroxidase levels, and parasite-specific IgG1 levels were lowered in IL-12 treated immunized animals, and parasite-specific IgG2a levels were increased in these animals. The data indicate that eosinophils are important as mediators of larval killing, and that the establishment of Th-2 type immunity results in killing of infective S. stercoralis L3, while a shift to Th-1 type immunity abrogates protective responses.
Volume
19
Issue
1
First Page
29
Last Page
39
ISSN
0141-9838
Published In/Presented At
Rotman, H. L., Schnyder-Candrian, S., Scott, P., Nolan, T. J., Schad, G. A., & Abraham, D. (1997). IL-12 eliminates the Th-2 dependent protective immune response of mice to larval Strongyloides stercoralis. Parasite immunology, 19(1), 29–39. https://doi.org/10.1046/j.1365-3024.1997.d01-142.x
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
9121838
Department(s)
Administration and Leadership
Document Type
Article