The c-MYC oncoprotein is a substrate of the acetyltransferases hGCN5/PCAF and TIP60.
Publication/Presentation Date
12-1-2004
Abstract
The c-MYC oncoprotein functions as a sequence-specific transcription factor. The ability of c-MYC to activate transcription relies in part on the recruitment of cofactor complexes containing the histone acetyltransferases mammalian GCN5 (mGCN5)/PCAF and TIP60. In addition to acetylating histones, these enzymes have been shown to acetylate other proteins involved in transcription, including sequence-specific transcription factors. This study was initiated in order to determine whether c-MYC is a direct substrate of mGCN5 and TIP60. We report here that mGCN5/PCAF and TIP60 acetylate c-MYC in vivo. By using nanoelectrospray tandem mass spectrometry to examine c-MYC purified from human cells, the major mGCN5-induced acetylation sites have been mapped. Acetylation of c-MYC by either mGCN5/PCAF or TIP60 results in a dramatic increase in protein stability. The data reported here suggest a conserved mechanism by which acetyltransferases regulate c-MYC function by altering its rate of degradation.
Volume
24
Issue
24
First Page
10826
Last Page
10834
ISSN
0270-7306
Published In/Presented At
Patel, J. H., Du, Y., Ard, P. G., Phillips, C., Carella, B., Chen, C. J., Rakowski, C., Chatterjee, C., Lieberman, P. M., Lane, W. S., Blobel, G. A., & McMahon, S. B. (2004). The c-MYC oncoprotein is a substrate of the acetyltransferases hGCN5/PCAF and TIP60. Molecular and cellular biology, 24(24), 10826–10834. https://doi.org/10.1128/MCB.24.24.10826-10834.2004
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
15572685
Department(s)
Administration and Leadership
Document Type
Article