Peroxynitrite aggravates myocardial reperfusion injury in the isolated perfused rat heart.
Publication/Presentation Date
11-1-1997
Abstract
OBJECTIVE: This study examined the effects of peroxynitrite (ONOO-) on cardiac function and cellular injury following ischemia (30 min) and reperfusion (60 min) in isolated perfused rat hearts.
METHODS: 3-Morpholinosydnonimine (SIN-1, 0.1 mM), an ONOO- donor, was administered alone or combined with superoxide dismutase (SOD, 300 U/ml) or glutathione (GSH, 1 mM) at the time of reperfusion.
RESULTS: Administration of SIN-1 alone significantly aggravated post-ischemic myocardial injury characterized by depressed cardiac function recovery (p < 0.05 vs. vehicle), increased lactic dehydrogenase (LDH) and creatine kinase (CK) release (p < 0.01 vs. vehicle), and enlarged necrotic size (p < 0.01 vs. vehicle). The co-administration of either SOD to decrease the formation of ONOO-, or GSH to increase the detoxification of ONOO-, completely blocked the detrimental effects of SIN-1 and exerted significant cardioprotective effects against reperfusion injury.
CONCLUSION: These results suggest that ONOO- may play a significant role in postischemic myocardial injury.
Volume
36
Issue
2
First Page
195
Last Page
204
ISSN
0008-6363
Published In/Presented At
Ma, X. L., Lopez, B. L., Liu, G. L., Christopher, T. A., & Ischiropoulos, H. (1997). Peroxynitrite aggravates myocardial reperfusion injury in the isolated perfused rat heart. Cardiovascular research, 36(2), 195–204. https://doi.org/10.1016/s0008-6363(97)00179-x
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
9463631
Department(s)
Administration and Leadership
Document Type
Article