Development of a New Zealand white rabbit model of spinal pseudarthrosis repair and evaluation of the potential role of OP-1 to overcome pseudarthrosis.

Authors

• Jonathan N Grauer
• Alexander R Vaccaro
• Masahiro Kato
• Brian K Kwon
• John M Beiner
• Tushar C Patel
• Alan S Hilibrand
• Kazuhiro Chiba
• Todd J Albert

Publication/Presentation Date

7-1-2004

Abstract

STUDY DESIGN: Posterolateral lumbar fusions were performed in nicotine-exposed, New Zealand white rabbits. Animals that developed a pseudarthrosis were then regrafted with no graft, autograft, or osteogenic protein-1 (OP-1).

OBJECTIVES: To establish a model of pseudarthrosis repair and to evaluate the ability of OP-1 to induce fusion in this model.

SUMMARY OF BACKGROUND DATA: OP-1 has been shown to have a 100% fusion rate in an established rabbit fusion model, even in the presence of nicotine, which is known to inhibit fusion.

METHODS: Forty-four New Zealand white rabbits underwent posterolateral lumbar fusion with iliac crest autograft. To maximize the incidence of pseudarthroses, nicotine was administered to all rabbits. At 5 weeks, the spines were explored, and all pseudarthroses were redecorticated and grafted with no graft, autograft, or OP-1. At 10 weeks, the rabbits were killed and fusions masses were assessed with manual palpation, radiography, computed tomography, and/or histology.

RESULTS: Nine rabbits (20%) were lost to complications. Thirty-four (94%) had pseudarthroses on exploration at 5 weeks. By manual palpation at 10 weeks, 1 of 10 (10%) pseudarthroses that received no graft fused, 5 of 12 (42%) pseudarthroses that received autograft fused, and 9 of 11 (82%) pseudarthroses that received OP-1 fused. Computed tomography and histology further characterized the fusion masses.

CONCLUSIONS: This study establishes a model for treatment of pseudarthroses. OP-1, which has previously been shown to have 100% fusion rate in animal models, outperformed autograft and induced fusion in 82% of rabbits.

Volume

29

Issue

13

First Page

1405

Last Page

1412

ISSN

1528-1159

Published In/Presented At

Grauer, J. N., Vaccaro, A. R., Kato, M., Kwon, B. K., Beiner, J. M., Patel, T. C., Hilibrand, A. S., Chiba, K., & Albert, T. J. (2004). Development of a New Zealand white rabbit model of spinal pseudarthrosis repair and evaluation of the potential role of OP-1 to overcome pseudarthrosis. Spine, 29(13), 1405–1412. https://doi.org/10.1097/01.brs.0000129028.25671.96

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

15223930

Department(s)

Administration and Leadership

Document Type

Article