Method for culturing postnatal substantia nigra as an in vitro model of experimental Parkinson's disease.
Publication/Presentation Date
4-1-2002
Abstract
One of the most prevalent degenerative disorders of the nervous system is Parkinson's disease. The etiology of this disease is for the most part unknown, although it is posited to arise from an interaction of genetic and environmental factors. Although in vivo animal studies have been used to examine the effects of a number of Parkinson-inducing compounds, there is little information on reliable in vitro methodologies that can recapitulate the previously observed in vivo results. Here, we describe a method for generating mixed and chimeric neuron/glial cultures of postnatal substantia nigra (SN), independent of other monoaminergic nuclei in the ventral midbrain. Since many toxins do not affect regions of the midbrain except the SN, use of whole ventral midbrain from embryos can dilute any measurement of cell death. By specifically culturing ventrolateral midbrain containing the substantia nigra, one can more directly target the effects of dopaminergic toxins. In addition, this method can be used to test potential therapies for amelioration of Parkinson's disease.
Volume
9
Issue
2
First Page
105
Last Page
111
ISSN
1385-299X
Published In/Presented At
Smeyne, M., & Smeyne, R. J. (2002). Method for culturing postnatal substantia nigra as an in vitro model of experimental Parkinson's disease. Brain research. Brain research protocols, 9(2), 105–111. https://doi.org/10.1016/s1385-299x(02)00117-4
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
12034329
Department(s)
Administration and Leadership
Document Type
Article