Local control of granule cell generation by cerebellar Purkinje cells.
Publication/Presentation Date
6-1-1995
Abstract
Cerebellar Purkinje cells were ablated by the specific expression of diphtheria toxin in these cells in transgenic mice. Purkinje cell degeneration during early postnatal development shows a zonally restricted pattern which has been exploited in order to look for local secondary effects. The most obvious early effect is the alignment of gaps in the Purkinje cell layer with dramatically thinned zones in the overlying EGL, the germinal layer from which granule cells are generated. Within these EGL zones in the transgenic mutant, markers that distinguish matrix from mantle cells demonstrate a preferential loss of the proliferative cells. Comparison of BrdU incorporation in the mutant vs wild-type confirms the reduction in proliferation. In the mutant, in situ labeling of DNA fragmentation associated with apoptotic cell death shows abundant labeling of granule cells that have exited the EGL, but not of progenitor cells in the EGL. Thus, although a trophic role for Purkinje cells has been well documented, these observations further suggest a mitogenic role which can be exerted locally.
Volume
6
Issue
3
First Page
230
Last Page
251
ISSN
1044-7431
Published In/Presented At
Smeyne, R. J., Chu, T., Lewin, A., Bian, F., Sanlioglu, S., Kunsch, C., Lira, S. A., & Oberdick, J. (1995). Local control of granule cell generation by cerebellar Purkinje cells. Molecular and cellular neurosciences, 6(3), 230–251. https://doi.org/10.1006/mcne.1995.1019
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
7496629
Department(s)
Administration and Leadership
Document Type
Article