Regulation of c-fos expression in transgenic mice requires multiple interdependent transcription control elements.
Publication/Presentation Date
2-1-1995
Abstract
Transcription control regions of eukaryotic genes contain multiple sequence elements proposed to function independently to regulate transcription. We developed transgenic mice carrying fos-lacZ fusion genes with clustered point mutations in each of several distinct regulatory sequences: the sis-inducible element, the serum response element, the fos AP-1 site, and the calcium/cAMP response element. Analysis of Fos-lacZ expression in the CNS and in cultured cells demonstrated that all of the regulatory elements tested were required in concert for tissue- and stimulus-specific regulation of the c-fos promoter. This implies that the regulation of c-fos expression requires the concerted action of multiple control elements that direct the assembly of an interdependent transcription complex.
Volume
14
Issue
2
First Page
241
Last Page
252
ISSN
0896-6273
Published In/Presented At
Robertson, L. M., Kerppola, T. K., Vendrell, M., Luk, D., Smeyne, R. J., Bocchiaro, C., Morgan, J. I., & Curran, T. (1995). Regulation of c-fos expression in transgenic mice requires multiple interdependent transcription control elements. Neuron, 14(2), 241–252. https://doi.org/10.1016/0896-6273(95)90282-1
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
7857636
Department(s)
Administration and Leadership
Document Type
Article