Isolation and characterization of macrophages from rat embryonic muscle culture.
Publication/Presentation Date
11-1-1992
Abstract
We have previously described the wide distribution of resident macrophages in normal rat skeletal muscle. In this study, we investigated the characteristics of the macrophages that occur in rat embryo muscle cultures. We showed that cells of monocyte-macrophage lineage are present in primary muscle cultures of rat embryos (18 days in gestation) and that these cells form morphologically and phenotypically heterogeneous populations, based on their reaction with monoclonal antibodies ED1, ED2, ED3, and OX43. Constitutively Ia+ cells with dendritic appearance were also observed. Furthermore, we established the procedure for isolation of macrophages from the primary muscle cultures. The isolated cells, mostly ED1+, expressed class I and CD4 antigens and bore complement (C3) receptors on their surfaces. The fact that cell of monocyte-macrophage lineage occur in the embryonic muscle suggests that during embryogenesis these cells may enter the developing muscle and give rise to a population of tissue-associated macrophages.
Volume
52
Issue
5
First Page
537
Last Page
544
ISSN
0741-5400
Published In/Presented At
Honda, H., Kimura, H., & Rostami, A. (1992). Isolation and characterization of macrophages from rat embryonic muscle culture. Journal of leukocyte biology, 52(5), 537–544. https://doi.org/10.1002/jlb.52.5.537
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
1431565
Department(s)
Administration and Leadership
Document Type
Article