Expression of 3G11 epitope defines subpopulations of regulatory T cells with different suppressive potency.
Publication/Presentation Date
8-15-2010
Abstract
3G11, a sialylated carbohydrate epitope on the disialoganglioside molecule, is expressed predominantly on the surface of mouse CD4(+) T cells. Our previous studies suggested that lack of the 3G11 molecule could be a new cell surface marker for regulatory CD4(+) T cells. In the present study, we explore the relationship between 3G11(-) and CD25(+) T cells, a well-defined, naturally occurring regulatory T cell population. We found that a large proportion of CD25(+)CD4(+) T cells lack expression of 3G11 and that more 3G11(-)CD4(+) T cells express Foxp3 compared to the 3G11(+)CD4(+) population. Based on 3G11 and CD25 expression we sorted four CD4(+) T cell subpopulations and tested their phenotypes. Among four CD25/3G11-related CD4(+) T cell subpopulations, CD25(+)3G11(-) T cells expressed the highest levels of Foxp3 and IL-10 and most efficiently inhibited mitogenic and antigen-specific immune responses in vitro and clinical EAE in vivo, while CD25(-)3G11(+) T cells produced a higher level of proinflammatory cytokines and enhanced autoimmune responses. Thus, among CD4(+)CD25(+) T cells, CD25(+)3G11(-) T cells represent a more effective Treg subpopulation than CD25(+)3G11(+) T cells.
Volume
295
Issue
1-2
First Page
66
Last Page
74
ISSN
1878-5883
Published In/Presented At
Zhao, Z., Ciric, B., Yu, S., Li, H., Yang, J., Kamoun, M., Zhang, G. X., & Rostami, A. (2010). Expression of 3G11 epitope defines subpopulations of regulatory T cells with different suppressive potency. Journal of the neurological sciences, 295(1-2), 66–74. https://doi.org/10.1016/j.jns.2010.04.019
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
20621800
Department(s)
Administration and Leadership
Document Type
Article