Construction of a novel virus that targets HIV-1-infected cells and controls HIV-1 infection.
Publication/Presentation Date
9-5-1997
Abstract
We describe a recombinant vesicular stomatitis virus lacking its glycoprotein gene and expressing instead the HIV-1 receptor CD4 and a coreceptor, CXCR4. This virus was unable to infect normal cells but did infect, propagate on, and kill cells that were first infected with HIV-1 and therefore had the HIV membrane fusion protein on their surface. Killing of HIV-1-infected cells controlled HIV infection in a T cell line and reduced titers of infectious HIV-1 in the culture by as much as 10(4)-fold. Such a targeted virus could have therapeutic value in reducing HIV viral load. Our results also demonstrate a general strategy of targeting one virus to the envelope protein of another virus to control infection.
Volume
90
Issue
5
First Page
849
Last Page
857
ISSN
0092-8674
Published In/Presented At
Schnell, M. J., Johnson, J. E., Buonocore, L., & Rose, J. K. (1997). Construction of a novel virus that targets HIV-1-infected cells and controls HIV-1 infection. Cell, 90(5), 849–857. https://doi.org/10.1016/s0092-8674(00)80350-5
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
9298897
Department(s)
Administration and Leadership
Document Type
Article