Rabies virus glycoprotein as a carrier for anthrax protective antigen.
Publication/Presentation Date
9-30-2006
Abstract
Live viral vectors expressing foreign antigens have shown great promise as vaccines against viral diseases. However, safety concerns remain a major problem regarding the use of even highly attenuated viral vectors. Using the rabies virus (RV) envelope protein as a carrier molecule, we show here that inactivated RV particles can be utilized to present Bacillus anthracis protective antigen (PA) domain-4 in the viral membrane. In addition to the RV glycoprotein (G) transmembrane and cytoplasmic domains, a portion of the RV G ectodomain was required to express the chimeric RV G anthrax PA on the cell surface. The novel antigen was also efficiently incorporated into RV virions. Mice immunized with the inactivated recombinant RV virions exhibited seroconversion against both RV G and anthrax PA, and a second inoculation greatly increased these responses. These data demonstrate that a viral envelope protein can carry a bacterial protein and that a viral carrier can display whole polypeptides compared to the limited epitope presentation of previous viral systems.
Volume
353
Issue
2
First Page
344
Last Page
356
ISSN
0042-6822
Published In/Presented At
Smith, M. E., Koser, M., Xiao, S., Siler, C., McGettigan, J. P., Calkins, C., Pomerantz, R. J., Dietzschold, B., & Schnell, M. J. (2006). Rabies virus glycoprotein as a carrier for anthrax protective antigen. Virology, 353(2), 344–356. https://doi.org/10.1016/j.virol.2006.05.010
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
16820183
Department(s)
Administration and Leadership
Document Type
Article