Human CD8+ cytotoxic T cell responses to adenovirus capsid proteins.
Publication/Presentation Date
7-5-2006
Abstract
Adenoviruses (Ads) cause fatal disease in allogeneic stem cell transplant recipients, but there is no established therapy. Ad-specific CD8+ T cells were detected in PBMC from healthy adults at a mean frequency of 77 per 10(5) CD8+ T cells (range 8-260) by interferon-gamma ELISPOT and cytokine flow cytometry assays. CD8+ T cell lines from 7 of 7 donors exhibited MHC-class-I-restricted killing of targets expressing the capsid protein hexon. In contrast, cytotoxicity against the capsid proteins fiber and penton base was weaker or not detected. Two HLA-A2-restricted hexon epitopes and one HLA-B-restricted epitope were identified, all of which are adjacent to or overlap an HLA-DP4-restricted epitope in the highly conserved C-terminus. Thus, hexon is the immunodominant T cell target among capsid proteins and contains multiple C-terminal epitopes conserved among serotypes. These data support evaluation of donor lymphocyte infusions for treatment of Ad disease post-transplant.
Volume
350
Issue
2
First Page
312
Last Page
322
ISSN
0042-6822
Published In/Presented At
Tang, J., Olive, M., Pulmanausahakul, R., Schnell, M., Flomenberg, N., Eisenlohr, L., & Flomenberg, P. (2006). Human CD8+ cytotoxic T cell responses to adenovirus capsid proteins. Virology, 350(2), 312–322. https://doi.org/10.1016/j.virol.2006.01.024
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
16499941
Department(s)
Administration and Leadership
Document Type
Article