siRNA targeting vaccinia virus double-stranded RNA binding protein [E3L] exerts potent antiviral effects.
Publication/Presentation Date
5-10-2006
Abstract
The Vaccinia virus gene, E3L, encodes a double-stranded RNA [dsRNA]-binding protein. We hypothesized that, owing to the critical nature of dsRNA in triggering host innate antiviral responses, E3L-specific small-interfering RNAs [siRNAs] should be effective antiviral agents against pox viruses, for which Vaccinia virus is an appropriate surrogate. In this study, we have utilized two human cell types, namely, HeLa and 293T, one which responds to interferon [IFN]-beta and the other produces and responds to IFN-beta, respectively. The antiviral effects were equally robust in HeLa and 293T cells. However, in the case of 293T cells, several distinct features were observed, when IFN-beta is activated in these cells. Vaccinia virus replication was inhibited by 97% and 98% as compared to control infection in HeLa and 293T cells transfected with E3L-specific siRNAs, respectively. These studies demonstrate the utility of E3L-specific siRNAs as potent antiviral agents for small pox and related pox viruses.
Volume
348
Issue
2
First Page
489
Last Page
497
ISSN
0042-6822
Published In/Presented At
Dave, R. S., McGettigan, J. P., Qureshi, T., Schnell, M. J., Nunnari, G., & Pomerantz, R. J. (2006). siRNA targeting vaccinia virus double-stranded RNA binding protein [E3L] exerts potent antiviral effects. Virology, 348(2), 489–497. https://doi.org/10.1016/j.virol.2006.01.013
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
16480752
Department(s)
Administration and Leadership
Document Type
Article