Cell-type-specific gene delivery into neuronal cells in vitro and in vivo.
Publication/Presentation Date
9-15-2003
Abstract
The avian retroviruses reticuloendotheliosis virus strain A (REV-A) and spleen necrosis virus (SNV) are not naturally infectious in human cells. However, REV-A-derived viral vectors efficiently infect human cells when they are pseudotyped with envelope proteins displaying targeting ligands specific for human cell-surface receptors. Here we report that vectors containing the gag region of REV-A and pol of SNV can be pseudotyped with the envelope protein of vesicular stomatitis virus (VSV) and the glycoproteins of different rabies virus (RV) strains. Vectors pseudotyped with the envelope protein of the highly neurotropic RV strain CVS-N2c facilitated cell type-specific gene delivery into mouse and human neurons, but did not infect other human cell types. Moreover, when such vector particles were injected into the brain of newborn mice, only neuronal cells were infected in vivo. Cell-type-specific gene delivery into neurons may present quite specific gene therapy approaches for many degenerative diseases of the brain.
Volume
314
Issue
1
First Page
74
Last Page
83
ISSN
0042-6822
Published In/Presented At
Parveen, Z., Mukhtar, M., Rafi, M., Wenger, D. A., Siddiqui, K. M., Siler, C. A., Dietzschold, B., Pomerantz, R. J., Schnell, M. J., & Dornburg, R. (2003). Cell-type-specific gene delivery into neuronal cells in vitro and in vivo. Virology, 314(1), 74–83. https://doi.org/10.1016/s0042-6822(03)00402-1
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
14517061
Department(s)
Administration and Leadership
Document Type
Article