A novel composite immunotoxin that suppresses rabies virus production by the infected cells.
Publication/Presentation Date
2-28-2010
Abstract
Using strepavidin as a scaffold, we have assembled a composite immunotoxin that consists of recombinant Pseudomonas exotoxin A subunit (PE38) and recombinant 25-D1.16 Fab fragment which recognizes the SIINFEKL (pOV8) peptide from ovalbumin in association with H-2K(b) MHC class I protein. The composite immunotoxin exercises cytotoxicity against H-2K(b+) cells sensitized with pOV8 peptide but not with irrelevant peptide. Specific binding of the immunotoxin to H-2K(b+) cells infected with recombinant rabies virus (RV) expressing pOV8 epitope (RV-pOV8) resulted in the suppression of the production of virus particles by the infected cells. This strategy allows readily produce different immunotoxins with desired specificity by combining various targeting and toxin molecules. The results provide a proof of concept that composite immunotoxins can be utilized as novel immunotherapeutics to stop virus spread in the acute phase of the infection allowing winning time for the development of protective immune response.
Volume
353
Issue
1-2
First Page
78
Last Page
86
ISSN
1872-7905
Published In/Presented At
Mareeva, T., Wanjalla, C., Schnell, M. J., & Sykulev, Y. (2010). A novel composite immunotoxin that suppresses rabies virus production by the infected cells. Journal of immunological methods, 353(1-2), 78–86. https://doi.org/10.1016/j.jim.2009.11.010
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
19932697
Department(s)
Administration and Leadership
Document Type
Article