Inactivated or live-attenuated bivalent vaccines that confer protection against rabies and Ebola viruses.
Publication/Presentation Date
10-1-2011
Abstract
The search for a safe and efficacious vaccine for Ebola virus continues, as no current vaccine candidate is nearing licensure. We have developed (i) replication-competent, (ii) replication-deficient, and (iii) chemically inactivated rabies virus (RABV) vaccines expressing Zaire Ebola virus (ZEBOV) glycoprotein (GP) by a reverse genetics system based on the SAD B19 RABV wildlife vaccine. ZEBOV GP is efficiently expressed by these vaccine candidates and is incorporated into virions. The vaccine candidates were avirulent after inoculation of adult mice, and viruses with a deletion in the RABV glycoprotein had greatly reduced neurovirulence after intracerebral inoculation in suckling mice. Immunization with live or inactivated RABV vaccines expressing ZEBOV GP induced humoral immunity against each virus and conferred protection from both lethal RABV and EBOV challenge in mice. The bivalent RABV/ZEBOV vaccines described here have several distinct advantages that may speed the development of inactivated vaccines for use in humans and potentially live or inactivated vaccines for use in nonhuman primates at risk of EBOV infection in endemic areas.
Volume
85
Issue
20
First Page
10605
Last Page
10616
ISSN
1098-5514
Published In/Presented At
Blaney, J. E., Wirblich, C., Papaneri, A. B., Johnson, R. F., Myers, C. J., Juelich, T. L., Holbrook, M. R., Freiberg, A. N., Bernbaum, J. G., Jahrling, P. B., Paragas, J., & Schnell, M. J. (2011). Inactivated or live-attenuated bivalent vaccines that confer protection against rabies and Ebola viruses. Journal of virology, 85(20), 10605–10616. https://doi.org/10.1128/JVI.00558-11
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
21849459
Department(s)
Administration and Leadership
Document Type
Article