Proliferating cell nuclear antigen is required for loading of the SMCX/KMD5C histone demethylase onto chromatin.
Publication/Presentation Date
10-13-2011
Abstract
BACKGROUND: Histone methylation is regulated by a large number of histone methyltransferases and demethylases. The recently discovered SMCX/KMD5C demethylase has been shown to remove methyl residues from lysine 4 of histone H3 (H3K4), and constitutes an important component of the regulatory element-1-silencing transcription factor (REST) protein complex. However, little is known about the cellular mechanisms that control SMCX activity and intracellular trafficking.
RESULTS: In this study, we found that small interfering RNA-mediated knockdown of proliferating cell nuclear antigen (PCNA) resulted in the reduction of the chromatin-bound SMCX fraction. We identified a PCNA-interaction protein motif (PIP box) in the SMCX protein. Using site-directed mutagenesis, we found that the amino acids of the SMCX PIP box are involved in the association of SMCX with PCNA and its interaction with chromatin.
CONCLUSIONS: Our data indicate that the intracellular trafficking of SMCX is controlled by its association with PCNA.
Volume
4
Issue
1
First Page
18
Last Page
18
ISSN
1756-8935
Published In/Presented At
Liang, Z., Diamond, M., Smith, J. A., Schnell, M., & Daniel, R. (2011). Proliferating cell nuclear antigen is required for loading of the SMCX/KMD5C histone demethylase onto chromatin. Epigenetics & chromatin, 4(1), 18. https://doi.org/10.1186/1756-8935-4-18
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
21996408
Department(s)
Administration and Leadership
Document Type
Article