Targeted single-neuron infection with rabies virus for transneuronal multisynaptic tracing.

Authors

• Tuan D Nguyen
• Christoph Wirblich
• Elias Aizenman
• Matthias J Schnell
• Peter L Strick
• Karl Kandler

Publication/Presentation Date

8-15-2012

Abstract

The transynaptic and retrograde spread of rabies virus make it an efficient and robust transneuronal tracer, capable of revealing connectivity patterns of multisynaptic, neuronal circuits with great detail. Current techniques begin by infecting many neurons simultaneously, from which higher-order neurons are then labeled sequentially in time. Here we report on a method that can initially infect a single neuron-of-choice, allowing for greater precision and specificity of labeled circuits.

Volume

209

Issue

2

First Page

367

Last Page

370

ISSN

1872-678X

Published In/Presented At

Nguyen, T. D., Wirblich, C., Aizenman, E., Schnell, M. J., Strick, P. L., & Kandler, K. (2012). Targeted single-neuron infection with rabies virus for transneuronal multisynaptic tracing. Journal of neuroscience methods, 209(2), 367–370. https://doi.org/10.1016/j.jneumeth.2012.06.019

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

22749814

Department(s)

Administration and Leadership

Document Type

Article