Manual and automated direct sequencing of product generated by the polymerase chain reaction.
Publication/Presentation Date
1-1-1993
Abstract
Identification of point mutations has been facilitated by a number of techniques, including transfection assays, oligonucleotide hybridization, electrophoretic migration of heteroduplexes, RNase mismatch analysis, direct sequencing, and DNA-polymerase catalyzed amplification. The large number of available techniques emphasizes the importance of developing rapid and reliable methods to identify molecular changes in genes. To date, we have concentrated on exploiting DNA-polymerase catalyzed amplification methods (1,2) in conjunction with direct manual and automated DNA sequencing to detect point mutations in the dihydrofolate reductase (DHFR) gene of methotrexate-resistant cells.
Volume
15
First Page
143
Last Page
152
ISSN
1064-3745
Published In/Presented At
Dicker, A. P., Volkenandt, M., & Bertino, J. R. (1993). Manual and automated direct sequencing of product generated by the polymerase chain reaction. Methods in molecular biology (Clifton, N.J.), 15, 143–152. https://doi.org/10.1385/0-89603-244-2:143
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
21400272
Department(s)
Administration and Leadership
Document Type
Article