Stereotactic Body Radiation Therapy Delivery in a Genetically Engineered Mouse Model of Lung Cancer.

Authors

• Shisuo Du
• Virginia Lockamy
• Lin Zhou
• Christine Xue
• Justin LeBlanc
• Shonna Glenn
• Gaurav Shukla
• Yan Yu
• Adam P Dicker
• Dennis B Leeper
• You Lu
• Bo Lu

Publication/Presentation Date

11-1-2016

Abstract

PURPOSE: To implement clinical stereotactic body radiation therapy (SBRT) using a small animal radiation research platform (SARRP) in a genetically engineered mouse model of lung cancer.

METHODS AND MATERIALS: A murine model of multinodular Kras-driven spontaneous lung tumors was used for this study. High-resolution cone beam computed tomography (CBCT) imaging was used to identify and target peripheral tumor nodules, whereas off-target lung nodules in the contralateral lung were used as a nonirradiated control. CBCT imaging helps localize tumors, facilitate high-precision irradiation, and monitor tumor growth. SBRT planning, prescription dose, and dose limits to normal tissue followed the guidelines set by RTOG protocols. Pathologic changes in the irradiated tumors were investigated using immunohistochemistry.

RESULTS: The image guided radiation delivery using the SARRP system effectively localized and treated lung cancer with precision in a genetically engineered mouse model of lung cancer. Immunohistochemical data confirmed the precise delivery of SBRT to the targeted lung nodules. The 60 Gy delivered in 3 weekly fractions markedly reduced the proliferation index, Ki-67, and increased apoptosis per staining for cleaved caspase-3 in irradiated lung nodules.

CONCLUSIONS: It is feasible to use the SARRP platform to perform dosimetric planning and delivery of SBRT in mice with lung cancer. This allows for preclinical studies that provide a rationale for clinical trials involving SBRT, especially when combined with immunotherapeutics.

Volume

96

Issue

3

First Page

529

Last Page

537

ISSN

1879-355X

Published In/Presented At

Du, S., Lockamy, V., Zhou, L., Xue, C., LeBlanc, J., Glenn, S., Shukla, G., Yu, Y., Dicker, A. P., Leeper, D. B., Lu, Y., & Lu, B. (2016). Stereotactic Body Radiation Therapy Delivery in a Genetically Engineered Mouse Model of Lung Cancer. International journal of radiation oncology, biology, physics, 96(3), 529–537. https://doi.org/10.1016/j.ijrobp.2016.07.008

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

27681749

Department(s)

Administration and Leadership

Document Type

Article