Transforming growth factor-beta inhibits the growth of renal cell carcinoma in vitro.
Publication/Presentation Date
5-1-1989
Abstract
Transforming growth factor-beta (TGF-beta) is a bifunctional growth regulatory hormone which inhibits the growth of many normal and neoplastic epithelial cell lines in monolayer culture. Endogenous and exogenous TGF-beta may influence cell proliferation through autocrine and paracrine binding to specific TGF-beta receptors. Growth effects of TGF-beta on human renal cell carcinoma cell lines have not been thus far described. We have studied the effects of TGF-beta on one renal tumor-derived (UOK-39) and one established (SKRC-7) renal cell carcinoma cell line. Exogenous addition of biologically active TGF-beta to cell cultures at concentrations between two and five ng./ml. inhibited the anchorage-dependent growth of UOK-39 by 75% and SKRC-7 by 44%, relative to controls. Low numbers of high affinity TGF-beta receptors were identified on both cell lines in 125I-TGF-beta binding assays. UOK-39 cells bound radiolabeled TGF-beta with higher affinity than SKRC-7 cells, but had fewer receptor sites, by Scatchard analysis of binding data. These results suggest that TGF-beta inhibits proliferation of renal carcinoma cells in vitro which may be mediated through binding of exogenous TGF-beta to functional TGF-beta receptors on the cell surface.
Volume
141
Issue
5
First Page
1240
Last Page
1244
ISSN
0022-5347
Published In/Presented At
Gomella, L. G., Sargent, E. R., Linehan, W. M., & Kasid, A. (1989). Transforming growth factor-beta inhibits the growth of renal cell carcinoma in vitro. The Journal of urology, 141(5), 1240–1244. https://doi.org/10.1016/s0022-5347(17)41230-4
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
2540353
Department(s)
Administration and Leadership
Document Type
Article