Assessment of isomiR Discrimination Using Commercial qPCR Methods.

Authors

• Rogan Magee
• Aristeidis G Telonis
• Tess Cherlin
• Isidore Rigoutsos
• Eric Londin

Publication/Presentation Date

6-1-2017

Abstract

We sought to determine whether commercial quantitative polymerase chain reaction (qPCR) methods are capable of distinguishing isomiRs: variants of mature microRNAs (miRNAs) with sequence endpoint differences. We used two commercially available miRNA qPCR methods to quantify miR-21-5p in both synthetic and real cell contexts. We find that although these miRNA qPCR methods possess high sensitivity for specific sequences, they also pick up background signals from closely related isomiRs, which influences the reliable quantification of individual isomiRs. We conclude that these methods do not possess the requisite specificity for reliable isomiR quantification.

Volume

3

Issue

2

ISSN

2311-553X

Published In/Presented At

Magee, R., Telonis, A. G., Cherlin, T., Rigoutsos, I., & Londin, E. (2017). Assessment of isomiR Discrimination Using Commercial qPCR Methods. Non-coding RNA, 3(2), 18. https://doi.org/10.3390/ncrna3020018

Disciplines

Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods

PubMedID

28730153

Department(s)

Administration and Leadership

Document Type

Article