Rat intestinal cell atrial natriuretic peptide receptor coupled to guanylate cyclase.
Publication/Presentation Date
7-1-1990
Abstract
The present studies were initiated to determine if cells of intestinal origin possess the molecular components supporting a response to atrial natriuretic peptides. Specific binding in cultured rat ileal cells with 125I-labeled atrial natriuretic peptide was saturable and of high affinity. Scatchard analyses showed a single population of binding sites with a Kd of 2.1 nmol/L and a Bmax of 300 fmol/mg protein. Atrial natriuretic peptide activated particulate guanylate cyclase 5- to 10-fold in a concentration- and time-dependent fashion. The EC50 for activation of enzyme by atrial natriuretic peptide was 6 nmol/L. Accumulation of cyclic guanosine monophosphate stimulated by atrial natriuretic peptide was observed in the intracellular (25-fold) and extracellular (50-fold) compartments and was dependent on concentration and time. Half-maximum intracellular accumulation was observed with 10 nmol/L atrial natriuretic peptide. These data suggest a role for atrial natriuretic peptides in the gastrointestinal tract.
Volume
99
Issue
1
First Page
125
Last Page
131
ISSN
0016-5085
Published In/Presented At
Crane, M. R., O'Hanley, P., & Waldman, S. A. (1990). Rat intestinal cell atrial natriuretic peptide receptor coupled to guanylate cyclase. Gastroenterology, 99(1), 125–131. https://doi.org/10.1016/0016-5085(90)91239-3
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
1971606
Department(s)
Administration and Leadership
Document Type
Article