Comparison of particulate guanylate cyclase in cells with and without atrial natriuretic peptide receptor binding activity.
Publication/Presentation Date
10-5-1989
Abstract
A line of kidney cells (PK1) which does not possess measurable ANP binding but has an active particulate guanylate cyclase has been identified. The physical characteristics of this enzyme were compared with those of particulate guanylate cyclase and ANP receptors isolated from rat lung. Although receptor and enzyme appear to reside on the same protein in the lung while the cyclase from PK1 cells does not possess ANP binding activity, these proteins exhibit identical physical characteristics. Guanylate cyclase from PK1 cells and rat lung and ANP receptor from lung co-eluted during gel filtration chromatography, with a Stokes radius of 6.1 nm. Also, these activities co-migrated through sucrose density gradients with S20,w values of 10.4 to 10.9. Using these parameters, a molecular weight of about 270 kD was estimated for all three activities. Furthermore, these enzyme activities exhibited similar mobilities in isoelectric focusing gels, with a pI of 6.1. Thus, although particulate guanylate cyclase from lung presumably possesses receptor binding activity, it is physically identical to a form of this enzyme associated with no measurable binding activity. Possible explanations for these observations are discussed.
Volume
90
Issue
1
First Page
19
Last Page
25
ISSN
0300-8177
Published In/Presented At
Waldman, S. A., Leitman, D. C., Chang, L. Y., & Murad, F. (1989). Comparison of particulate guanylate cyclase in cells with and without atrial natriuretic peptide receptor binding activity. Molecular and cellular biochemistry, 90(1), 19–25. https://doi.org/10.1007/BF00225217
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
2575208
Department(s)
Administration and Leadership
Document Type
Article