A splice variant of the transcript for guanylyl cyclase C is expressed in human colon and colorectal cancer cells.
Publication/Presentation Date
2-1-2000
Abstract
Guanylyl cyclase C is a sensitive and specific biomarker for metastatic colorectal cancer. A variant of the guanylyl cyclase C transcript was identified that possesses a 142-bp deletion at the 3' end of exon 1 reflecting alternative splicing of mRNA, introducing a shift in the open reading frame that prevents translation of a guanylyl cyclase C-related product. This variant was identified in human intestine and colon carcinomas, but not in extraintestinal tissues or tumors. These studies demonstrate that GCC and the splice variant contribute to the pool of GCC transcripts detected by RT-PCR in human tissues. They indicate that primers for RT-PCR that amplify regions downstream from the deletion are required to assess the full complement of GCC transcripts (GCC + GCC(var)) in human tissues and body fluids for staging and postoperative surveillance of patients with colorectal cancer.
Volume
45
Issue
2
First Page
298
Last Page
305
ISSN
0163-2116
Published In/Presented At
Pearlman, J. M., Prawer, S. P., Barber, M. T., Parkinson, S. J., Schulz, S., Park, J., Zook, M., & Waldman, S. A. (2000). A splice variant of the transcript for guanylyl cyclase C is expressed in human colon and colorectal cancer cells. Digestive diseases and sciences, 45(2), 298–305. https://doi.org/10.1023/a:1005456408768
Disciplines
Business Administration, Management, and Operations | Health and Medical Administration | Management Sciences and Quantitative Methods
PubMedID
10711442
Department(s)
Administration and Leadership
Document Type
Article