m-Azipropofol (AziPm) a photoactive analogue of the intravenous general anesthetic propofol.

Publication/Presentation Date

8-12-2010

Abstract

Propofol is the most commonly used sedative-hypnotic drug for noxious procedures, yet the molecular targets underlying either its beneficial or toxic effects remain uncertain. In order to determine targets and thereby mechanisms of propofol, we have synthesized a photoactivateable analogue by substituting an alkyldiazirinyl moiety for one of the isopropyl arms but in the meta position. m-Azipropofol retains the physical, biochemical, GABA(A) receptor modulatory, and in vivo activity of propofol and photoadducts to amino acid residues in known propofol binding sites in natural proteins. Using either mass spectrometry or radiolabeling, this reagent may be used to reveal sites and targets that underlie the mechanism of both the desirable and undesirable actions of this important clinical compound.

Volume

53

Issue

15

First Page

5667

Last Page

5675

ISSN

1520-4804

Disciplines

Anesthesiology | Medicine and Health Sciences

PubMedID

20597506

Department(s)

Department of Anesthesiology

Document Type

Article

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