A novel approach employing ultrasound guidance for percutaneous cardiac muscle injection to retrograde label rat stellate ganglion neurons.
Publication/Presentation Date
6-17-2004
Abstract
Stellate ganglion (SG) neurons provide the main sympathetic innervation to the heart and help to regulate cardiac function. The purpose of this study was to determine if ultrasound imaging could be employed to retrograde label rat SG neurons innervating the heart without employing thoracotomy. In addition, electrophysiological experiments were performed to characterize the modulation of Ca(2+) channels by neurotransmitters in unlabeled and dye-labeled SG neurons. Fluorescence imaging of actutely isolated cells revealed that dye uptake was successful within five days following injection of dye in the cardiac muscle. Whole-cell voltage-clamp recordings revealed that the majority of the Ca(2+) current was carried by N-type Ca(2+) channels. Finally, fluorescence dye uptake did not appear to affect the modulation of Ca(2+) currents following exposure of SG neurons to norepinephrine, adenosine and neurokinin A. These results demonstrate that ultrasound imaging-guided percutaneous injection can be effectively employed to retrograde label neurons innervating the heart.
Volume
363
Issue
3
First Page
252
Last Page
256
ISSN
0304-3940
Published In/Presented At
Fuller, B. C., Sumner, A. D., Kutzler, M. A., & Ruiz-Velasco, V. (2004). A novel approach employing ultrasound guidance for percutaneous cardiac muscle injection to retrograde label rat stellate ganglion neurons. Neuroscience letters, 363(3), 252–256. https://doi.org/10.1016/j.neulet.2004.03.087
Disciplines
Medicine and Health Sciences
PubMedID
15182954
Department(s)
Department of Medicine, Cardiology Division
Document Type
Article