A recapitulative three-dimensional model of breast carcinoma requires perfusion for multi-week growth.

Publication/Presentation Date

1-1-2016

Abstract

Breast carcinomas are complex, three-dimensional tissues composed of cancer epithelial cells and stromal components, including fibroblasts and extracellular matrix. In vitro models that more faithfully recapitulate this dimensionality and stromal microenvironment should more accurately elucidate the processes driving carcinogenesis, tumor progression, and therapeutic response. Herein, novel in vitro breast carcinoma surrogates, distinguished by a relevant dimensionality and stromal microenvironment, are described and characterized. A perfusion bioreactor system was used to deliver medium to surrogates containing engineered microchannels and the effects of perfusion, medium composition, and the method of cell incorporation and density of initial cell seeding on the growth and morphology of surrogates were assessed. Perfused surrogates demonstrated significantly greater cell density and proliferation and were more histologically recapitulative of human breast carcinoma than surrogates maintained without perfusion. Although other parameters of the surrogate system, such as medium composition and cell seeding density, affected cell growth, perfusion was the most influential parameter.

Volume

7

First Page

2041731416660739

Last Page

2041731416660739

ISSN

2041-7314

Disciplines

Dentistry | Medicine and Health Sciences

PubMedID

27516850

Department(s)

Department of Dental Medicine, Department of Dental Medicine Residents, Fellows and Residents

Document Type

Article

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