Suspension matrices for improved Schwann-cell survival after implantation into the injured rat spinal cord.

Publication/Presentation Date

5-1-2010

Abstract

Trauma to the spinal cord produces endogenously irreversible tissue and functional loss, requiring the application of therapeutic approaches to achieve meaningful restoration. Cellular strategies, in particular Schwann-cell implantation, have shown promise in overcoming many of the obstacles facing successful repair of the injured spinal cord. Here, we show that the implantation of Schwann cells as cell suspensions with in-situ gelling laminin:collagen matrices after spinal-cord contusion significantly enhances long-term cell survival but not proliferation, as well as improves graft vascularization and the degree of axonal in-growth over the standard implantation vehicle, minimal media. The use of a matrix to suspend cells prior to implantation should be an important consideration for achieving improved survival and effectiveness of cellular therapies for future clinical application.

Volume

27

Issue

5

First Page

789

Last Page

801

ISSN

1557-9042

Disciplines

Medicine and Health Sciences

PubMedID

20144012

Department(s)

Department of Emergency Medicine

Document Type

Article

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