Digital Polymerase Chain Reaction Paired with High-Speed Atomic Force Microscopy for Quantitation and Length Analysis of DNA Length Polymorphisms.
Publication/Presentation Date
11-24-2020
Abstract
DNA length polymorphisms are found in many serious diseases, and assessment of their length and abundance is often critical for accurate diagnosis. However, measuring their length and frequency in a mostly wild-type background, as occurs in many situations, remains challenging due to their variable and repetitive nature. To overcome these hurdles, we combined two powerful techniques, digital polymerase chain reaction (dPCR) and high-speed atomic force microscopy (HSAFM), to create a simple, rapid, and flexible method for quantifying both the size and proportion of DNA length polymorphisms. In our approach, individual amplicons from each dPCR partition are imaged and sized directly. We focused on internal tandem duplications (ITDs) located within the
Volume
14
Issue
11
First Page
15385
Last Page
15393
ISSN
1936-086X
Published In/Presented At
Koebley, S. R., Mikheikin, A., Leslie, K., Guest, D., McConnell-Wells, W., Lehman, J. H., Al Juhaishi, T., Zhang, X., Roberts, C. H., Picco, L., Toor, A., Chesney, A., & Reed, J. (2020). Digital Polymerase Chain Reaction Paired with High-Speed Atomic Force Microscopy for Quantitation and Length Analysis of DNA Length Polymorphisms. ACS nano, 14(11), 15385–15393. https://doi.org/10.1021/acsnano.0c05897
Disciplines
Medicine and Health Sciences
PubMedID
33169971
Department(s)
Department of Medicine, Hematology-Medical Oncology Division
Document Type
Article