Whole exome sequencing to estimate alloreactivity potential between donors and recipients in stem cell transplantation.
Publication/Presentation Date
8-1-2014
Abstract
Whole exome sequencing (WES) was performed on stem cell transplant donor-recipient (D-R) pairs to determine the extent of potential antigenic variation at a molecular level. In a small cohort of D-R pairs, a high frequency of sequence variation was observed between the donor and recipient exomes independent of human leucocyte antigen (HLA) matching. Nonsynonymous, nonconservative single nucleotide polymorphisms were approximately twice as frequent in HLA-matched unrelated, compared with related D-R pairs. When mapped to individual chromosomes, these polymorphic nucleotides were uniformly distributed across the entire exome. In conclusion, WES reveals extensive nucleotide sequence variation in the exomes of HLA-matched donors and recipients.
Volume
166
Issue
4
First Page
566
Last Page
570
ISSN
1365-2141
Published In/Presented At
Sampson, J. K., Sheth, N. U., Koparde, V. N., Scalora, A. F., Serrano, M. G., Lee, V., Roberts, C. H., Jameson-Lee, M., Ferreira-Gonzalez, A., Manjili, M. H., Buck, G. A., Neale, M. C., & Toor, A. A. (2014). Whole exome sequencing to estimate alloreactivity potential between donors and recipients in stem cell transplantation. British journal of haematology, 166(4), 566–570. https://doi.org/10.1111/bjh.12898
Disciplines
Medicine and Health Sciences
PubMedID
24749631
Department(s)
Department of Medicine, Hematology-Medical Oncology Division
Document Type
Article