Sequence homology between HLA-bound cytomegalovirus and human peptides: A potential trigger for alloreactivity.

Publication/Presentation Date

1-1-2017

Abstract

Human cytomegalovirus (hCMV) reactivation may often coincide with the development of graft-versus-host-disease (GVHD) in stem cell transplantation (SCT). Seventy seven SCT donor-recipient pairs (DRP) (HLA matched unrelated donor (MUD), n = 50; matched related donor (MRD), n = 27) underwent whole exome sequencing to identify single nucleotide polymorphisms (SNPs) generating alloreactive peptide libraries for each DRP (9-mer peptide-HLA complexes); Human CMV CROSS (Cross-Reactive Open Source Sequence) database was compiled from NCBI; HLA class I binding affinity for each DRPs HLA was calculated by NetMHCpan 2.8 and hCMV- derived 9-mers algorithmically compared to the alloreactive peptide-HLA complex libraries. Short consecutive (≥6) amino acid (AA) sequence homology matching hCMV to recipient peptides was considered for HLA-bound-peptide (IC50

Volume

12

Issue

8

First Page

0178763

Last Page

0178763

ISSN

1932-6203

Disciplines

Medicine and Health Sciences

PubMedID

28800601

Department(s)

Department of Medicine, Hematology-Medical Oncology Division

Document Type

Article

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