An improved method for in vitro susceptibility testing of Trichomonas vaginalis.
Publication/Presentation Date
1-1-1995
Abstract
A protocol is presented for in vitro susceptibility testing of Trichomonas vaginalis. A 100 ml culture of the microorganisms is prepared for inoculation into antibiotic dilutions and controls by centrifugation, washing with 10 mM HEPES (pH 6.2) plus 1.5 x 10(-1) M NaCl, a second centrifugation and a resuspension in the HEPES-saline buffer. Inclusion of the gelling agent carrageenan in the culture medium permits an ease of harvesting the trichomonads and a reproducible initial cell density of 1-4 x 10(4) cells per ml. Following inoculation, tubes with antibiotic dilutions and controls are incubated anaerobically at 35 degrees C for 48 h, which corresponds to late exponential phase. Inclusion of a negative control helps determine Minimum Lethal Concentration (MLC) values.
Volume
390
First Page
211
Last Page
216
ISSN
0065-2598
Published In/Presented At
Bromke, B. J., Furiga, M. C., Hendershot, R. C., & McGinn, M. (1995). An improved method for in vitro susceptibility testing of Trichomonas vaginalis. Advances in experimental medicine and biology, 390, 211–216. https://doi.org/10.1007/978-1-4757-9203-4_18
Disciplines
Medicine and Health Sciences
PubMedID
8718615
Department(s)
Department of Medicine
Document Type
Article