Urinary Single-Cell Profiling Captures the Cellular Diversity of the Kidney.
Publication/Presentation Date
3-1-2021
Abstract
BACKGROUND: Microscopic analysis of urine sediment is probably the most commonly used diagnostic procedure in nephrology. The urinary cells, however, have not yet undergone careful unbiased characterization.
METHODS: Single-cell transcriptomic analysis was performed on 17 urine samples obtained from five subjects at two different occasions, using both spot and 24-hour urine collection. A pooled urine sample from multiple healthy individuals served as a reference control. In total 23,082 cells were analyzed. Urinary cells were compared with human kidney and human bladder datasets to understand similarities and differences among the observed cell types.
RESULTS: Almost all kidney cell types can be identified in urine, such as podocyte, proximal tubule, loop of Henle, and collecting duct, in addition to macrophages, lymphocytes, and bladder cells. The urinary cell-type composition was subject specific and reasonably stable using different collection methods and over time. Urinary cells clustered with kidney and bladder cells, such as urinary podocytes with kidney podocytes, and principal cells of the kidney and urine, indicating their similarities in gene expression.
CONCLUSIONS: A reference dataset for cells in human urine was generated. Single-cell transcriptomics enables detection and quantification of almost all types of cells in the kidney and urinary tract.
Volume
32
Issue
3
First Page
614
Last Page
627
ISSN
1533-3450
Published In/Presented At
Abedini, A., Zhu, Y. O., Chatterjee, S., Halasz, G., Devalaraja-Narashimha, K., Shrestha, R., Balzer, M. S., Park, J., Zhou, T., Ma, Z., Sullivan, K. M., Hu, H., Sheng, X., Liu, H., Wei, Y., Boustany-Kari, C. M., Patel, U., Almaani, S., Palmer, M., Townsend, R., … TRIDENT Study Investigators (2021). Urinary Single-Cell Profiling Captures the Cellular Diversity of the Kidney. Journal of the American Society of Nephrology : JASN, 32(3), 614–627. https://doi.org/10.1681/ASN.2020050757
Disciplines
Medicine and Health Sciences
PubMedID
33531352
Department(s)
Department of Medicine
Document Type
Article