Ethanol administration to cystic fibrosis knockout mice results in increased fatty acid ethyl ester production.
Publication/Presentation Date
11-1-2005
Abstract
BACKGROUND: Fatty acid ethyl esters (FAEE) are nonoxidative ethanol metabolites shown to produce toxic effects in the liver and pancreas in vivo and in vitro. Because alcohol-induced chronic pancreatitis is associated with mutations in the gene responsible for cystic fibrosis (CFTR), we hypothesized that CFTR dysfunction leads to increased levels of these toxic nonoxidative ethanol metabolites following alcohol administration.
METHODS: Cystic fibrosis (CF) and wild-type (WT) mice were injected intraperitoneally with 1, 2, or 3 g/kg of 50% ethanol. Mice were sacrificed and the liver and pancreas removed for FAEE analysis.
RESULTS: The mean FAEE concentration (pmol/g) detected in the liver of cftr mice following injection with 2 g/kg of ethanol was significantly greater than the amount detected in WT (p < 0.005). A similar trend in FAEE concentration was seen in the pancreas, but the difference was not statistically different. In both the liver and pancreas, analysis of individual FAEE species demonstrated a selective increase in ethyl oleate.
CONCLUSION: These data show an association between CFTR dysfunction and qualitative and quantitative changes in FAEE in liver and pancreas upon ethanol exposure.
Volume
29
Issue
11
First Page
2039
Last Page
2045
ISSN
0145-6008
Published In/Presented At
Blanco, P. G., Salem, R. O., Ollero, M., Zaman, M. M., Cluette-Brown, J. E., Freedman, S. D., & Laposata, M. (2005). Ethanol administration to cystic fibrosis knockout mice results in increased fatty acid ethyl ester production. Alcoholism, clinical and experimental research, 29(11), 2039–2045. https://doi.org/10.1097/01.alc.0000187593.86202.e8
Disciplines
Medicine and Health Sciences
PubMedID
16340462
Department(s)
Department of Medicine
Document Type
Article