Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application.
Publication/Presentation Date
12-1-2005
Abstract
INTRODUCTION: Semiquantitative evaluation of liver specimens is considered the standard method for measuring fibrosis; however, these systems lack the precision of a quantitative technique.
METHODS: We developed an image analysis application (FibroXact) that automates and simplifies color segmentation. Trichrome slides were scanned and the program was used to evaluate each pixel based upon hue, lightness, and saturation values. Percent fibrosis was automatically calculated after thresholding.
RESULTS: Linear regression demonstrated a strong relationship between the FibroXact result and the semiquantitative result (P<0.001).
CONCLUSION: We have developed an image analysis application to quantify liver fibrosis. Correlation between our results and a standard semiquantitative system was demonstrated.
Volume
25
Issue
6
First Page
1156
Last Page
1161
ISSN
1478-3223
Published In/Presented At
Friedenberg, M. A., Miller, L., Chung, C. Y., Fleszler, F., Banson, F. L., Thomas, R., Swartz, K. P., & Friedenberg, F. K. (2005). Simplified method of hepatic fibrosis quantification: design of a new morphometric analysis application. Liver international : official journal of the International Association for the Study of the Liver, 25(6), 1156–1161. https://doi.org/10.1111/j.1478-3231.2005.01161.x
Disciplines
Medicine and Health Sciences
PubMedID
16343066
Department(s)
Department of Medicine
Document Type
Article