Pentagastrin-stimulated gastric acid secretion by the isolated perfused rat stomach.
Publication/Presentation Date
6-18-1984
Abstract
The purpose of this present study was to develop a method for stimulation of acid secretion by the isolated perfused rat stomach. Rat stomachs were perfused in situ via the abdominal aorta and celiac axis with Krebs-Ringer bicarbonate buffer in the presence or absence of 10% ovine erythrocytes. The gastric lumen was perfused with distilled water and gastric contents were collected at frequent intervals through a catheter at the pylorus. Sixty minute gastric acid output in response to various concentrations of pentagastrin was determined by titration of gastric contents with 0.01 N NaOH to pH 7.0. During arterial perfusion with Krebs-Ringer bicarbonate buffer in the absence of ovine erythrocytes gastric acid output was 2.50 +/- 0.58 SEM microEq H+/h, which did not increase in response to perfusion with Krebs-Ringer bicarbonate buffer containing pentagastrin. However, inclusion of 10% ovine erythrocytes in the arterial perfusate resulted in substantial stimulation of gastric acid by pentagastrin: maximal acid output, achieved with a pentagastrin dose of 0.6 microgram/kg/h, was 23.5 +/- 3.73 microEq H+/h (p less than 0.01). The results of the present study demonstrate the capacity of the isolated vascularly perfused rat stomach to secrete acid and provide a model for studying interactions of gastrointestinal regulatory peptides and their physiologic roles in the regulation of gastric acid secretion.
Volume
34
Issue
25
First Page
2515
Last Page
2523
ISSN
0024-3205
Published In/Presented At
Short, G. M., Wolfe, M. M., & McGuigan, J. E. (1984). Pentagastrin-stimulated gastric acid secretion by the isolated perfused rat stomach. Life sciences, 34(25), 2515–2523. https://doi.org/10.1016/0024-3205(84)90289-3
Disciplines
Medicine and Health Sciences
PubMedID
6727580
Department(s)
Department of Medicine
Document Type
Article