The effect of cigarette smoke versus vaporized nicotine on healing of a rat femur.
Publication/Presentation Date
10-1-2022
Abstract
INTRODUCTION: Little data exists regarding the effects of vaporized nicotine on healing. Our goal was to compare vaporized nicotine, combusted nicotine and control with respect to bone healing in a rat femur fracture model.
MATERIALS AND METHODS: Forty-five male Sprague Dawley rats were divided into three equal cohorts. Rats were exposed to two cigarettes daily, an equivalent dose of vaporized nicotine, or control, six days a week. Exposures occurred for 4 weeks prior to iatrogenic femur fracture and intramedullary repair. Four additional weeks of exposure occurred prior to sacrifice. Radiographic, biomechanical and histologic analysis was conducted.
RESULTS: No significant difference between the three groups was identified for total mineralized bone volume (p = 0.14), total volume of mature bone (p = 0.12) or immature bone (p = 0.15). Importantly, less total mineralized bone volume and immature bone volume was seen in the vaporized nicotine group compared to combusted tobacco, but results were not significant. Biomechanical testing revealed no significant difference in group torsional stiffness (p = 0.92) or maximum torque (p = 0.31) between the three groups. On histologic analysis, chi-square testing showed no significant difference in any category.
CONCLUSIONS: This exploratory study compared combusted nicotine, vaporized nicotine and a control on rat femur fractures. While no statistically significant differences were identified, there were trends showing less total mineralized bone volume and immature bone volume in the vaporized nicotine group compared to the other groups. Additional study is warranted based on our findings.
Volume
53
Issue
10
First Page
3102
Last Page
3108
ISSN
1879-0267
Published In/Presented At
Tucker, J., McCullen, A., Kennedy, P., Koroneos, Z., Wee, H. B., Dhawan, A., Atkins, H., Lewis, G. S., & Garner, M. R. (2022). The effect of cigarette smoke versus vaporized nicotine on healing of a rat femur. Injury, 53(10), 3102–3108. https://doi.org/10.1016/j.injury.2022.08.040
Disciplines
Medicine and Health Sciences
PubMedID
36030094
Department(s)
Department of Medicine, Department of Medicine Fellows and Residents, Fellows and Residents
Document Type
Article