Comparative genome analysis of potential regulatory elements in the ABCG5-ABCG8 gene cluster.
Publication/Presentation Date
7-12-2002
Abstract
The excretion of sterols from the liver and intestine is regulated by the ABCG5 and ABCG8 transporters. To identify potential regulatory elements, 152 kb of the human ABCG5-ABCG8 gene cluster was sequenced and comparative genome analysis was performed. The two genes are oriented in a head-to-head configuration and are separated by a 374-bp intergenic region, which is highly conserved among several species. Using a reporter construct, the intergenic region was found to act as a bidirectional promoter. A conserved GATA site in the intergenic region was shown by site-directed mutagenesis to act as a repressor for the ABCG5 promoter. The intergenic region was also shown to be partially responsive to treatment by LXR agonists. In summary, several potential regulatory elements were found for the ABCG5 and ABCG8 genes, and the intergenic region was found to act as a bidirectional promoter.
Volume
295
Issue
2
First Page
276
Last Page
282
ISSN
0006-291X
Published In/Presented At
Remaley AT, Bark S, Walts AD, Freeman L, Shulenin S, Annilo T, Elgin E, Rhodes HE, Joyce C, Dean M, Santamarina-Fojo S, Brewer HB Jr. Comparative genome analysis of potential regulatory elements in the ABCG5-ABCG8 gene cluster. Biochem Biophys Res Commun. 2002 Jul 12;295(2):276-82. doi: 10.1016/s0006-291x(02)00652-6. PMID: 12150943.
Disciplines
Medicine and Health Sciences
PubMedID
12150943
Department(s)
Department of Medicine, Cardiology Division
Document Type
Article