Exercise and lymphocyte activation following chemotherapy for breast cancer.
Publication/Presentation Date
11-1-2005
Abstract
PURPOSE: To determine whether exercise training would increase lymphocyte activation in patients with breast cancer following chemotherapy. Activation was determined by the presence of CD4(+)CD69(+) T-helper lymphocytes, mitogen-induced proliferation, and levels of cytokines produced by mitogen-stimulated lymphocytes and in the patients' plasma.
METHODS: Patients with breast cancer (N = 28) who participated in a 6-month exercise program were compared with patients (N = 21) who did not exercise. Following chemotherapy, and 3 and 6 months later, patients underwent fitness evaluations and had blood drawn. The exercise program consisted of resistance training and aerobic activity at 60-75% functional capacity three times a week with a personal trainer. Immunochemistry and flow cytometry were used to measure the number of CD4(+)CD69(+) blood lymphocytes. Whole blood was stimulated with concanavalin A (ConA), phytohemagglutin (PHA), or pokeweed mitogen (PWM) to determine proliferation potential. Enzyme-linked immunosorbent assays (ELISA) were used to determine the concentration of interferon-gamma (IFN-gamma) and interleukin-6 (IL-6) in the culture medium of mitogen-stimulated lymphocytes as well as the plasma concentrations of IL-6, soluble IL-6 receptor, soluble gp130, and IFN-gamma. Analysis of groups across time was done using the Wilcoxon signed rank test, and comparisons of groups were done using the Mann-Whitney U test.
RESULTS: The exercising patients showed increases in maximal oxygen uptake and upper body strength. This group also showed a greater percentage of CD4(+)CD69(+) cells and a greater level of tritiated thymidine incorporation (DNA synthesis) when stimulated with ConA, PHA, and PWM at the end of the intervention. Plasma and mitogen-stimulated IL-6 and IFN-gamma production were similar in both groups.
CONCLUSION: Exercise may improve immune function by increasing lymphocyte activation in patients with breast cancer following treatment.
Volume
37
Issue
11
First Page
1827
Last Page
1835
ISSN
0195-9131
Published In/Presented At
Hutnick, N. A., Williams, N. I., Kraemer, W. J., Orsega-Smith, E., Dixon, R. H., Bleznak, A. D., & Mastro, A. M. (2005). Exercise and lymphocyte activation following chemotherapy for breast cancer. Medicine and science in sports and exercise, 37(11), 1827–1835. https://doi.org/10.1249/01.mss.0000175857.84936.1a
Disciplines
Medicine and Health Sciences
PubMedID
16286849
Department(s)
Department of Medicine
Document Type
Article