Cord blood rescues stroke-induced changes in splenocyte phenotype and function.
Publication/Presentation Date
5-1-2006
Abstract
The neuroprotective mechanism of human umbilical cord blood cells (HUCBC) in the rat middle cerebral artery occlusion (MCAO) stroke model remains uncertain. Given the inflammatory sequelae that occur following stroke, we investigated whether HUCBC protection could be derived from the modulation of this immuno-inflammatory event, suggested by the attraction of the HUCBC to the spleen. We found that, following MCAO, rat spleen size was reduced concomitantly with their CD8+ T-cell counts. Interestingly, MCAO-induced spleen size reduction correlated with the extent of ischemic damage, however, HUCBC treatment rescued the spleen weight, splenic CD8+ T-cell counts, as well as the amount of brain injury. Additionally, splenocyte proliferation assays demonstrated that HUCBC treatment opposed MCAO-associated T-cell proliferation by increasing the production of IL-10 while decreasing IFN-gamma. Taken together, these results suggest a novel immunomodulatory mechanism by which HUCBC mediate protection in the rat MCAO model of stroke.
Volume
199
Issue
1
First Page
191
Last Page
200
ISSN
0014-4886
Published In/Presented At
Vendrame, M., Gemma, C., Pennypacker, K. R., Bickford, P. C., Davis Sanberg, C., Sanberg, P. R., & Willing, A. E. (2006). Cord blood rescues stroke-induced changes in splenocyte phenotype and function. Experimental neurology, 199(1), 191–200. https://doi.org/10.1016/j.expneurol.2006.03.017
Disciplines
Medicine and Health Sciences
PubMedID
16713598
Department(s)
Department of Medicine
Document Type
Article