Participation of the bacteriophage Mu A protein and host factors in the initiation of Mu DNA synthesis in vitro.
Publication/Presentation Date
6-10-1994
Abstract
During bacteriophage Mu transposition, strand transfer is catalyzed in the presence of phage-encoded A and B proteins and Escherichia coli HU protein, attaching Mu ends to target DNA and creating an intermediate in transposition. Bacteriophage Mu A protein, which remains tightly bound to the Mu ends in the native strand-transfer intermediate, blocked initiation of Mu DNA replication by a system of 8 host proteins (DnaB helicase, DnaC protein, DnaG primase, DNA polymerase III holoenzyme, DNA polymerase I, DNA gyrase, DNA ligase, and single-strand binding protein). This 8-protein system had all enzymatic activities to convert the deproteinized intermediate to a cointegrate; however, additional host factor(s) were required to replicate the native intermediate. While replication of the native intermediate absolutely required DnaB helicase, DnaC protein, and DNA polymerase III holoenzyme, the specific requirements were relaxed for the deproteinized intermediate. Other host factors were able to replace these specific factors. These results indicate that Mu A protein, in conjunction with additional host factor(s), acts to promote assembly of specific host replication proteins at the Mu replication fork. This process may alter the stable interaction of Mu A protein with the ends to allow initiation of Mu DNA synthesis.
Volume
269
Issue
23
First Page
16469
Last Page
16477
ISSN
0021-9258
Published In/Presented At
Kruklitis, R., & Nakai, H. (1994). Participation of the bacteriophage Mu A protein and host factors in the initiation of Mu DNA synthesis in vitro. The Journal of biological chemistry, 269(23), 16469–16477.
Disciplines
Medicine and Health Sciences
PubMedID
8206956
Department(s)
Department of Medicine
Document Type
Article