Characterization of astrocyte plasminogen activator.
Publication/Presentation Date
9-1-1987
Abstract
We have previously shown that astrocytes produce and secrete plasminogen activator (PA) and that this function is responsive to various modulating agents. When astrocyte conditioned medium (CM) is subjected to SDS-PAGE and PA activity localized by fibrin-agar gel overlay, the activity in the CM is found to comigrate with control t-PA. On affinity chromatography CM PA specifically binds to t-PA antibody. The latter also inhibits fibrinolytic activity of CM PA. When incubated with a fibrin clot, CM PA activity can be shown to bind to fibrin. These observations help identify the enzyme in astrocyte CM as t-PA. A possible role of astrocyte PA in myelin injury could provide an explanation for the previously observed correlation between fibrin deposition and demyelination as well as inhibition of demyelination by ancrod and heparin in experimental allergic encephalomyelitis.
Volume
80
Issue
2-3
First Page
277
Last Page
287
ISSN
0022-510X
Published In/Presented At
Toshniwal, P. K., Firestone, S. L., Barlow, G. H., & Tiku, M. L. (1987). Characterization of astrocyte plasminogen activator. Journal of the neurological sciences, 80(2-3), 277–287. https://doi.org/10.1016/0022-510x(87)90162-6
Disciplines
Medicine and Health Sciences
PubMedID
3119779
Department(s)
Department of Medicine
Document Type
Article