Genetic determinants of 5-lipoxygenase transcription.
Publication/Presentation Date
1-1-1999
Abstract
BACKGROUND: 5 Lipoxygenase (5-LO) is a critical enzyme in the production of the leukotrienes. We have identified a series of mutations in the 5-LO gene that modify gene transcription. These mutations consist of addition of an Sp-1 binding motif (-GGGCGG-) or deletion of one or two Sp-1 binding motifs in the 5-LO core promoter.
METHODS: Mutant forms of the 5-LO core promoter were placed in a chloramphenicol acetyl transferase (CAT) reporter construct using either HeLa or SL-2 cells.
RESULTS: In HeLa cells all of the mutant forms are less effective in driving CAT reporter activity than the wild-type promoter. In SL-2 cells the construct containing the addition mutation was more effective in driving CAT reporter activity, while the constructs containing the deletion mutations were less effective.
CONCLUSIONS: These data indicate that naturally occurring mutations in the 5-LO core promoter modify gene transcription in vitro.
Volume
118
Issue
2-4
First Page
275
Last Page
278
ISSN
1018-2438
Published In/Presented At
Drazen, J. M., & Silverman, E. S. (1999). Genetic determinants of 5-lipoxygenase transcription. International archives of allergy and immunology, 118(2-4), 275–278. https://doi.org/10.1159/000024098
Disciplines
Medicine and Health Sciences
PubMedID
10224409
Department(s)
Department of Medicine
Document Type
Article