Vascular smooth muscle cells express the transcriptional corepressor NAB2 in response to injury.
Publication/Presentation Date
10-1-1999
Abstract
The early growth response 1 (Egr-1 or NGFI-A) gene product is a zinc finger protein transcription factor which has been implicated in the regulation of genes differentially expressed during the development of vascular disease. Egr-1 activity is regulated by alterations in the amount of protein, as well as protein-protein interactions with positive and negative transcriptional cofactors. NGFI-A-binding protein 2 (NAB2) is an example of a negative transcriptional cofactor capable of binding directly to Egr-1 and repressing Egr-1-mediated transcription. In this study, we show that NAB2 is rapidly and transiently expressed in vascular smooth muscle cells (VSMC) in response to the model agonist phorbol 12-myristate 13-acetate (PMA). This induction occurs at the protein as well as mRNA level, and the time course of induction trails closely behind that of Egr-1. NAB2 expression in VSMC is capable of inhibiting Egr-1 dependent gene expression in response to either PMA or fibroblastic growth factor-2 (FGF-2). In an in vivo model of mechanical arterial injury NAB2 levels also increase transiently in VSMC at a time when Egr-1 is elevated. It is possible that NAB2 is part of a negative-feedback mechanism which serves to down-regulate Egr-1-mediated gene transcription in injured VSMC.
Volume
155
Issue
4
First Page
1311
Last Page
1317
ISSN
0002-9440
Published In/Presented At
Silverman, E. S., Khachigian, L. M., Santiago, F. S., Williams, A. J., Lindner, V., & Collins, T. (1999). Vascular smooth muscle cells express the transcriptional corepressor NAB2 in response to injury. The American journal of pathology, 155(4), 1311–1317. https://doi.org/10.1016/S0002-9440(10)65233-9
Disciplines
Medicine and Health Sciences
PubMedID
10514413
Department(s)
Department of Medicine
Document Type
Article